We have evaluated the accuracy of high sensitive CRP assay method using
evanescent wave Immunoassay system(Evanet 20) and significance of this
assay on the early detection of infectious diseases in newborn babies.
In this assay system, prozone phenomenon was not detected up to 40mg/dl.
The reproducibility of this assay was quite good and the intra run CV value
of the same sample was less than 5% for the assay of serum, plasma and
whole blood. There was a high correlation between the CRP values in the
serum and plasma(r＝0.98, regression formula y＝0.89x＋4.07). Similarly,
the values in whole blood and serum samples were quite well correlated(r＝0.98,
regression formula y＝0.91x−6.75). Various humoral elements such as bilirubin,
hemoglobin and Chyl did not significantly influence this assay method.
A slight increase in blood CRP was clearly demonstrated in the early phase
of infectious diseases of newborn babies and monitoring of CRP by this
assay system seemed to be quite useful to detect the early phase of infectious
diseases in newborn babies. This assay system requires only a small quantity
of whole blood to perform quantitative analysis of very small amounts of
other substances. Accordingly, this assay system seems to be quite effective
for monitoring minute increases in various proteinaceous blood components
in emergent laboratory examination or POCT.
[Rinsho Byori 50 : 24〜29, 2002]
*1Central Clinical Laboratory, Shiga University of Medical Science, Otsu 520-2192
*1滋賀医科大学医学部附属病院検査部，*2同 臨床検査医学講座(〒520-2192 大津市瀬田月輪町)
E-mail :tkotsuki@belle.shiga-med.ac.jp