HPLC 法による胸水ヒアルロン酸の定量
−従来から使用されている電気泳動法との比較−

藤　田　　　寛*1　前　田　　　浩*2　佐　倉　義　幸*3
　　　　　　　　　　　　　　　　　　　　　　
Application of HPLC Method for the Measurement of Hyaluronan in Pleural Fluid
−A New Alternative of Cellulose Acetate Membrane Electrophoresis−

Hiroshi FUJITA*1, Hiroshi MAEDA*2 and Yoshiyuki SAKURA*3

We examined whether high-performance liquid chromatography(HPLC) method was more suitable for the measurement of hyaluronan(HA) in pleural fluid than the standardized electrophoretical method. Forty-four samples of pleural fluid were measured both by the HPLC method and by the electrophoretical method. The HA values measured by the HPLC method did not show significant correlation(r＝0.33) to those measured by the electrophoretical method. We investigated the reason of this insufficiency of the correlation. The electrophoretical method was not specific for the measurement of HA and other glycosaminoglycan, such as chondroitin could be measured by this method. Therefore, the HA value measured by this method was higher than that measured by the HPLC method. The other problem was some loss of HA during the pre-treatment of samples with percloric acid in the electrophoretical method, which was needless in the HPLC method. We concluded that the HPLC method was more appropriate than the electrophoretical method for the measurement of hyaluronan in pleural fluid.
[Rinsho Byori 50 : 74〜78, 2002]

*1Glycoconjugate Research Center, Seikagaku Corp., Yokosuka 239-0831

受付2001年7月26日・受理2001年10月12日
*1〜3生化学工業�葛v里浜工場 糖鎖分析センター(〒239-0831 横須賀市久里浜7-3-1)

E-mail :fujita2@seikagaku.co.jp