ポストゲノムに関する諸問題(2)

cDNA マイクロアレイ技術の臨床検査への応用：
遺伝子発現プロファイル解析による癌の薬剤耐性形質の評価

宮　地　勇　人*1　小　林　広　幸*2　浅　井　さとみ*3
荒　見　真一郎*4　新　畑　智　也*5　三　瀬　静　男*6
　　　　　　　　　　　　　　　　　　　　　　
cDNA Microarray Technology as a Laboratory Examination Method:
A Gene Expression Profiling Test for Analysis of Drug Resistance in Tumor Cells

Hayato MIYACHI, MD*1, Hiroyuki KOBAYASHI, MD*2, Satomi ASAI, MD*3,
Tomoya SHINBATA*4, Shinichiro ARAMI*5 and Shizuo MITSUSE*6

cDNA microarray technology permits the simultaneous measurement of the expressions of thousands of genes. The technology is now an indispensable research tool in molecular biology, and the challenge is its development and usage as clinical diagnostic tools. cDNA microarray can be used to identify gene expression profiles in tumor cells which correlate strongly with the treatment responsiveness such as drug resistance and clinical outcome of the disease despite similar phenotypes. For the introduction of cDNA microarray into laboratory examination, many issues need to be resolved. Design of a diagnostic array must be developed with defined sequences based on interpretation of huge quantities of data from experimental arrays to predict treatment responsiveness. Assay quality must be improved in terms of detection sensitivity, reproducibility, and linear dynamic range for RNA quantitation. Generally available instruments, which are much less expensive and more practical, need to be developed. Along with the improvement of the assay as a laboratory examination method, cDNA microarray will facilitate the integration of diagnosis and therapeutics, and the introduction of individual medicines.
[Rinsho Byori 50 : 161〜168, 2002]

*1Department of Laboratory Medicine, Tokai University School of Medicine, Isehara 259-1193

【Key Words】cDNA microarray(cDNAマイクロアレイ)，laboratory examination(臨床検査)，gene expres-sion(遺伝子発現)，cancer(癌)

*1,3東海大学医学部臨床検査医学，*2同　医学部薬理学(〒259-1193 伊勢原市望星台)
*4〜6日本製粉中央研究所(〒243-0041 厚木市緑ヶ丘5-1-3)

E-mail :miyachi@is.icc.u-tokai.ac.jp