ポストゲノムに関する諸問題(3)

質量分析の新分野：プロテオミクスによる臨床研究

清　水　　　章*1　中　西　豊　文*2　小　山　玲　子*3　池　田　恒　彦*4
　　　　　　　　　　　　　　　　　　　　　　
Proteomics in Clinical Research : New Approach of Mass Spectrometry

Akira SHIMIZU, MD*1, Toyohumi NAKANISHI, PhD*2,
Reiko KOYAMA, MD*3 and Tsunehiko IKEDA, MD*4

A proteome has been defined as the protein complement expressed by the genome of an organism, tissue, or differentiated cell. Knowledge of complete genome sequences has led to considerable effort being increasingly devoted to the large-scale study of proteomes, that is, 'proteomics'. Commonly, two proteomes are compared by a substructive analysis in which differences due to drug treatment, culture conditions, genetic variations, or diseases can be observed. Two-dimensional gel electrophoresis and mass spectrometry are commonly used for this purpose.
We applied this approach to the analysis of vitreous humor(VH) proteins. Fifty-two different proteins were identified on silver-stained 2D-gel patterns with VH proteins obtained from diabetic retinopathy and macular hole. Thirty-five proteins, which have not reported in plasma, were found in VH. Pigment epithelium-derived factor, which was reported to be a potent inhibitor of angiogenesis in cornea and vitre-ous was at a higher concentration in VH with diabetes than in that with macular hole. It is impressive that the inhibitor increases in the vitreous with proliferative angiogenesis.
Unique applications in proteomics promise a bright future for molecular biology and hopefully for clini-cal chemistry.
[Rinsho Byori 50 : 169〜172, 2002]

*1Department of Clinical Pathology, Osaka Medical College, Takatsuki 569-8686

【Key Words】Two-dimensional gel electrophoresis/mass spectrometry(二次元電気泳動/質量分析)，Pro-teomics(プロテオミクス)，vitreous humor(硝子体)，Diabetic retinopathy(糖尿病網膜症)

*1,2大阪医科大学病態検査学，*3,4同　眼科(〒569-8686 高槻市大学町2-7)

E-mail :shimizu@poh.osaka-med.ac.jp