非等モル PSA(前立腺特異抗原)測定系を抗フリー PSA モノク
ローナル抗体添加により等モル測定系に改善する試み

木　村　恵　美*1　松　本　恭　一*2　佐　守　友　博*3
　　　　　　　　　　　　　　　　　　　　　　
Improvement of Total Prostate Specific Antigen(PSA) Assay from
the "Skewed Molar-Response" to the "Equimolar-Response" by
Utilizing anti Free-PSA Monoclonal Antibodies

Emi KIMURA*1, Kyoichi MATSUMOTO*2 and Tomohiro SAMORI, MD*3

Prostate specific antigen(PSA) is widely used as a marker for screening and monitoring prostate cancer. However, there are several assay methods, which often give different PSA values for the same patient sample. A possible cause of these discrepancies might be the varied immunoreactivity with free-PSA and complex-PSA among methods.
This study revealed that pre-treatment of samples with anti-free-PSA monoclonal antibodies improved polyclonal antibodies-based non equimolar PSA assay to equimolar assay.
This might be achieved by that the monoclonal antibodies bind to free-PSA and then change its reactivities with polyclonal antibodies to those similar to complex-PSA.
[Rinsho Byori 50 : 202〜206, 2002]

*1Japan Clinical Laboratories, Inc. Kuze-gun, Kyoto-pref. 613-0046

【Key Words】prostate specific antigen：PSA(前立腺特異抗原)，free-PSA(遊離型 PSA)，complex-PSA(結合型 PSA)，enzyme-linked immunosorbent assay：ELISA(酵素免疫測定法)，equimolar assay(等モル反応系)

受付2001年8月29日・受理2001年11月15日
*1〜3�M日本医学臨床検査研究所学術研究課(〒613-0046 京都府久世郡久御山町大橋辺16-10)

E-mail :e.kimura@lnm.jcl.co.jp