高脂血症の現状と検査(2)

HDL-C ホモジニアスアッセイの開発

杉　内　博　幸*1　岡　部　紘　明*2
　　　　　　　　　　　　　　　　　　　　　　
Development of Homogeneous Assay for HDL-C in Serum

Hiroyuki SUGIUCHI, PhD*1 and Hiroaki OKABE, MD*2

Recent development of several homogeneous assays for measuring HDL-cholesterol(HDL-C) in serum without pretreatment has been reviewed. Several problems on the five homogeneous assays based on PEG-modified enzyme/α-cyclodextrin sulfate(PEGME), polyanion-polymer/detergent(PPD), immunoinhibition, catalase/detergent and 4-sulfonyl calyx[8]areine are discussed comparing with CDC reference method. The two homogeneous assays(PEGME and PPD) used in many laboratories were precise and correlated well with CDC reference method(mean percentage bias : ＜5%). The discrepancies of recoveries for apo E-rich HDL-C were observed among reagents of homogeneous assays in serum from patients with hepatobiliary diseases and cholesterol ester transfer protein(CETP) deficiency. Apo E-rich HDL slightly contained in normal serum was most recovered by homogeneous methods, but not by precipitation methods. The differences between the normal values for HDL-C obtained by homogeneous assay(PEGME method) and precipitation method(dextran sulfate-phosphotungstate-MgCl2) were statistically significant(p＜0.01).
[Rinsho Byori 50 : 226〜233, 2002 ]

*1Department of Laboratory Medicine, Kumamoto University Hospital, Kumamoto 860-8556

【Key Words】cholesterol(コレステロール)，high-density lipoprotein：HDL，homogeneous assay(ホモジニアスアッセイ)，apo E-rich HDL(アポ E リッチ HDL)

*1熊本大学医学部附属病院中央検査部，*2同　医学部臨床検査医学講座(〒860-8556 熊本市本荘1-1-1)

E-mail :sugiuchi@gpo.kumamoto-u.ac.jp