DICの診断基準と血液凝固検査の標準化(3)

アンチトロンビン測定とその標準化

岡　嶋　研　二*
　　　　　　　　　　　　　　　　　　　　　　
Standardization of the Laboratory Method for Antithrombin Activity Measurement

Kenji OKAJIMA, MD*

Measurement of antithrombin(AT) activity in plasma provides important information for the diagnosis of congenital antithrombin deficiency and disseminated intravascular coagulation. When measured in the presence of heparin, plasma antithrombin activity includes heparin cofactor II activity as about 20〜30% of the total activity. Antithrombin assay methods measuring plasma anti-F.Xa activity or those measured in the presence of 0.22M NaCl exclude the influence of HC II activity. Dilution of the plasma sample is necessary to measure the heparin cofactor anti-thrombin activity of AT correctly. A laboratory method that precisely reflects plasma heparin cofactor anti-thrombin activity of AT should be established as the standard laboratory method.
[Rinsho Byori 50 : 283〜286, 2002]

*Department of Laboratory Medicine, Kumamoto University School of Medicine, Kumamoto 860-0811

【Key Words】antithrombin(アンチトロンビン)，congenital antithrombin deficiency(先天性アンチトロンビン欠損症)，disseminated intravascular coagulation(播種性血管内凝固症候群)，heparin cofactor II(ヘパリンコファクター II)，factor Xa(活性型凝固第 X 因子)

*熊本大学医学部臨床検査医学講座(〒860-8556 熊本市本荘1-1-1)

E-mail :whynot@kaiju.medic.kumamoto-u.ac.jp