[Rinsho Byori 50 : 308`312, 2002]

Determination of Medullasin Levels for the Diagnosis of Multiple Sclerosis

Yosuke AOKI, MD*1, Takahiko SAIDA, MD*2, Imaharu NAKANO, MD*3, Toyokazu SAITO, MD*4,
Kunihiko IKEGUCHI, MD*5, Takao URABE, MD*6, Etsuko NISHIGUCHI*7,
Hideaki SUZUKI, PhD*8, Kiyoshi TAKAHASHI*9 and Yoshikuni MIZUNO, MD*10

Medullasin levels in granulocytes of patients with neurological diseases and healthy volunteers were determined by the enzyme immunoassay using mouse monoclonal antibodies against human medullasin and o-phenylenediamine-H2O2 as the detection system of the enzyme activity. One hundred twenty-one out of 159 patients with multiple sclerosis(76.1%) showed positive results(above means of normals{2SD) in this test, while only 16.9%(24/142) of patients with non-inflammatory neurological diseases had positive results. This enzyme immunoassay method for medullasin is considered to be an useful paraclinical test for the diagnosis of multiple sclerosis.

*1Department of Food and Health Science, Faculty of Human Life Science, Jissen Women's University, Hino 191-8510

yKey Wordszmedullasin, multiple sclerosis, enzyme immunoassay, granulocytes