気管支，気管支肺胞洗浄液について考える−手技から病態解明まで−(3)

抗炎症分子クララ細胞10-kDa蛋白質と肺疾患

四十坊　典　晴*1　伊　藤　喜　久*2　阿　部　庄　作*3
　　　　　　　　　　　　　　　　　　　　　　
Anti-inflammatory Molecule, Clara Cell 10 Kilodalton Protein and Respiratory Diseases

Noriharu SHIJUBO, MD*1, Yoshihira ITOH, MD*2 and Shosaku ABE, MD*3

Clara cell 10 kilodalton protein(CC10) is the major product of non-ciliated bronchiolar epithelial cells (Clara cells) and is identical with uteroglobin and urinary protein 1(P-1). CC10/uteroglobin is a homodimer consisting of 70 amino acid subunits arranged in an antiparallel fashion and connected by two disulfide bonds. CC10/uteroglobin shows an anti-inflammatory property including inhibition of phospholipase A2 and phospholipase C. We analyzed the epitope of monoclonal antibodies(mAbs) to human CC10/P-1. TY-5, TY-7 and TY-8 recognized α1-, α2- and α3-helix of CC10, respectively. The combination of TY-1 and TY-2 are most suitable for ELISA, while TY-5 and TY-7 are very good for immunoblot and immunohistochemical analysis. Circulating CC10 levels are increased in sarcoidosis and decreased in asthma. Bronchoalveolar lavage CC10 levels are increased in sarcoidosis patients with a good outcome. The use of these mAbs is a powerful tool to investigate the clinical roles of CC10. CC10 is a regulator of the inflammatory process in respiratory diseases.
[Rinsho Byori 50 : 370〜373, 2002]

*1Third Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo 060-8543

【Key Words】Clara cell 10-kilodalton protein(クララ細胞 10-kDa 蛋白質)，uteroglobin(ウテログロビン)，asthma(喘息)，sarcoidosis(サルコイドーシス)

*1,3札幌医科大学医学部第三内科(〒060-8543 札幌市中央区南1条西16丁目)
*2旭川医科大学臨床検査医学(〒078-8510 旭川市緑が丘東2条1-1-1)

E-mail :shijubo@sapmed.ac.jp