臨床検査−最近の進歩−(2)

ラテックス試薬を用いた汎用自動分析装置による
血中エラスターゼ 1 の測定

大　出　勝　也*1　沢　井　時　男*2
　　　　　　　　　　　　　　　　　　　　　　
Determination of Elastase 1 in Plasma or Serum by
Latex Turbidometric Immunoassay with Automatic Analyzer

Katsuya OHDE*1 and Tokio SAWAI*2

We developed a latex turbidometric immunoassay(LTIA) for elastase 1. The precisions were 1.0〜2.8% intra-run and 1.2〜2.9% inter-day. The dilution test showed good linearity between 80〜4000ng/dl. There was interference by bilirubin, hemoglobin, lipemia or rheumatoid factor. Serum and plasma elastase 1 measured by LTIA( y ) showed significant correlations with those by RIA( x )(serum : y＝0.98x−18, r＝0.980, n＝64, plasma : y＝0.96x−19, r＝0.976, n＝61). It was confirmed that the values were elevated in patients with pancreatic disease compared to the normal level.
These results demonstrate that the determination of elastase 1 in plasma or serum by this method is useful, simple and time-saving, when compared with that by RIA.
[Rinsho Byori 50 : 376〜380, 2002]

*1Iatron Laboratories, INC., Chiba 289-2247

【Key Words】Elastase 1(エラスターゼ 1)，latex turbidometric immunoassay(ラテックス免疫比濁法)，pancreatic disease(膵疾患)

*1,2�潟сgロン研究開発本部(〒289-2247 千葉県香取郡多古町水戸字水戸台1460-6)

E-mail :ode@iatron.co.jp