We developed a latex turbidometric immunoassay(LTIA) for elastase 1. The precisions were 1.0〜2.8% intra-run and 1.2〜2.9% inter-day. The dilution test showed good linearity between 80〜4000ng/dl. There was interference by bilirubin, hemoglobin, lipemia or rheumatoid factor. Serum and plasma elastase 1 measured by LTIA( y ) showed significant correlations with those by RIA( x )(serum : y=0.98x-18, r=0.980, n=64, plasma : y=0.96x-19, r=0.976, n=61). It was confirmed that the values were elevated in patients with pancreatic disease compared to the normal level.
These results demonstrate that the determination of elastase 1 in plasma or serum by this method is useful, simple and time-saving, when compared with that by RIA.

* 1Iatron Laboratories, INC., Chiba 289-2247

【Key Words】 Elastase 1, latex turbidometric immunoassay, pancreatic disease