検査法の開発と病態解明へのアプローチ(6)

新しい腎障害の尿中マーカー，ATP
−抗癌剤投与患者，糖尿病患者，新生児での検討−

中　島　基　雄*1　江　藤　由　紀*2　矢　内　　　充*3　川　村　理佐子*4
金　子　一　成*5　青　柳　一　正*6　小　磯　健　吉*7　　　　　　　　
　　　　　　　　　　　　　　　　　　　　　　
Novel Renal Function Marker, ATP−Establishing the Normal Range, Cases of
Anti-Tumor Drags Administration for Urinary-Track Tumor,
Diabetic Diseases and a Newborn Baby

Moto-o NAKAJIMA*1, Yuki ETOH, MD*2, Mitsuru YANAI, MD*3,
Risako KAWAMURA, MD*4, Kazunari KANEKO, MD*5,
Kazumasa AOYAGI, MD*6 and Kenkiti KOISO, MD*7

Urinary free ATP assay by the firefly luciferin-luciferase method is a rapid and simple method for determining renal function, especially uriniferous tubule function. Normal range of urinary free ATP concentration, daily ATP excretion in urine, urinary ATP/creatinine value and ATP decomposition activity in urine is 1.1×10⁻⁹〜3.4×10⁻⁸M, 4.0×10⁻⁹〜4.1×10⁻⁸ mole, 5.0×10⁻¹³〜5.9×10⁻¹¹ mol/mgCr and 100〜77% express for the remaining rate of additional ATP, respectively. A significant correlation was found between free ATP concentration and daily ATP excretion in urine with a correlation coefficient of 0.84. In cases of anti-tumor drug(cisplatin＝cis-diamminedichloroplatinum II) administration for urinary-track tumor, abnormal urinary free ATP concentration and ATP decomposition activity in urine were clearly demonstrated after a few days of cisplatin administration. The appearance of a tendency toward abnormal relative ATP values were similar to changes in β₂-MG and NAG values. Diabetic patients often demonstrate unusually high values of urinary free ATP concentration. In asphyxia of the newborn, urinary ATP/creatinine value were significantly higher than those in healthy newborn, but urinary NAG values did not differ.
[Rinsho Byori 50 : 513〜518, 2002]

*1Research & Development Division, Kikkoman Co., Noda 278-0037

【Key Words】urinary free ATP(尿中遊離 ATP)，firefly luciferin-luciferase method(螢ルシフェリン-ルシフェラーゼ法)，renal function(腎機能)，uriniferous tubule function(尿細管機能)，case of anti-tumor drag administration(抗癌剤投与症例)，diabetic diseases patient(糖尿病患者)，asphyxia of the newborn(仮死新生児)
　　　　　　　　　　　　　　　　　　　　　
*1キッコーマン�褐､究本部研究推進部(〒278-0037 野田市野田399)
*2,3日本大学医学部臨床検査医学(〒173-8610 東京都板橋区大谷口上町30-1)
*4,5順天堂大学医学部小児科学(〒113-8421 東京都文京区本郷2-1-1)
*6筑波技術短大付属診療所(〒305-8575 つくば市天王台1-1-1)
*7せんぽ東京高輪病院(〒108-8606 東京都港区高輪3-10-11)

E-mail :nakajima@ttp.or.jp