[Rinsho Byori 50 : 513〜518, 2002]

Novel Renal Function Marker, ATP-Establishing the Normal Range, Cases of
Anti-Tumor Drags Administration for Urinary-Track Tumor,
Diabetic Diseases and a Newborn Baby

Moto-o NAKAJIMA*1, Yuki ETOH, MD*2, Mitsuru YANAI, MD*3,
Risako KAWAMURA, MD*4, Kazunari KANEKO, MD*5,
Kazumasa AOYAGI, MD*6 and Kenkiti KOISO, MD*7

Urinary free ATP assay by the firefly luciferin-luciferase method is a rapid and simple method for determining renal function, especially uriniferous tubule function. Normal range of urinary free ATP concentration, daily ATP excretion in urine, urinary ATP/creatinine value and ATP decomposition activity in urine is 1.1×10^-9〜3.4×10^-8M, 4.0×10^-9〜4.1×10^-8 mole, 5.0×10^-13〜5.9×10^-11 mol/mgCr and 100〜77% express for the remaining rate of additional ATP, respectively. A significant correlation was found between free ATP concentration and daily ATP excretion in urine with a correlation coefficient of 0.84. In cases of anti-tumor drug(cisplatin＝cis-diamminedichloroplatinum II) administration for urinary-track tumor, abnormal urinary free ATP concentration and ATP decomposition activity in urine were clearly demonstrated after a few days of cisplatin administration. The appearance of a tendency toward abnormal relative ATP values were similar to changes in β₂-MG and NAG values. Diabetic patients often demonstrate unusually high values of urinary free ATP concentration. In asphyxia of the newborn, urinary ATP/creatinine value were significantly higher than those in healthy newborn, but urinary NAG values did not differ.


*1Research & Development Division, Kikkoman Co., Noda 278-0037

【Key Words】urinary free ATP, firefly luciferin-luciferase method, renal function, uriniferous tubule function, case of anti-tumor drag administration, diabetic diseases patient, asphyxia of the newborn