臨床酵素学の進歩−血清酵素異常症へのアプローチ(3)

腫瘍産生アミラーゼ

森　山　隆　則*1　戸　沢　辰　雄*2　松　野　一　彦*3
　　　　　　　　　　　　　　　　　　　　　　
Tumor Producing Amylase

Takanori MORIYAMA, PhD*1, Tatsuo TOZAWA, MD*2
and Kazuhiko MATSUNO, MD*3

The phenotype of tumor-producing amylase is usually salivary-type amylase, in contrast, we experienced sialyl salivary-type amylase detected in the sera of the patients with malignancies. Sialyl salivary-type amylase, with an abnormal anodic migration, was detected by isoamylase electrophoretic analysis in the sera of patients with multiple myeloma and with ovarian cancer. The abnormal isoamylase bands migrated toward the anode faster than the salivary isoamylase band(S2) and were stained more strongly than S2 band. The isoamylases could be separated from residual normal isoamylases in the sera of patients by using gel permeation chromatography. The isoamylases were showed sensitive by neuraminidase treatment and were reacted with anti-human salivary monoclonal antibody.
[Rinsho Byori 50 : 566〜570, 2002]

*1Department of Laboratory Technology, College of Medical Technology, Hokkaido University, Sapporo 060-0812

【Key Words】sialyl salivary-type amylase(シアリル S 型アミラーゼ)，abnormal fast-migrated isoamylases(速易動の異常アイソアミラーゼ)，hyperamylasemia(高アミラーゼ血症)，myeloma(ミエローマ)，ovarian cancer(卵巣癌)

*1,3北海道大学医療技術短期大学部衛生技術学科(〒060-0812 札幌市北区北12 条西5丁目)
*2 兵庫医科大学臨床病理学(〒663-8501 西宮市武庫川町1番1号)

E-mail :moriyama@cme.hokudai.ac.jp