The measurement of CHO and TG concentrations in three lipoprotein subfractions(VLDL, LDL, and HDL) are useful to estimate qualitative change of lipoproteins. This method, CHO and TG dual staining using agarose gel electrophoresis(AG-CHO, TG staining), is conventional and the results correlated highly with ultracentrifugation. Using this method, we measured CHO and TG concentrations of VLDL, LDL, and HDL subfractions in 244 patients after an overnight fast. All cases were stratified to 4 groups, normolipidemia(LDL-CHO＜160mg/dl, sTG＜150mg/dl, n＝111), type IIa(n＝34), IIb(n＝39), and IV(n＝36), according to hyperlipidemia types. Furthermore, normolipidemia with low HDL cases(HDL-CHO＜45mg/dl, n＝24) distinguished from HDL-CHO≧45mg/dl, normolipidemia cases(n＝111). Between serum TCHO and LDL-CHO, serum TG and VLDL-CHO, TG showed positive correlation(r＝0.895, 0.971), particularly serum TG and VLDL-TG showed strong positive in all lipidemia types. However, serum TG and VLDL-CHO was strongly positive in type IIb and IV(r＝0.825, 0.823), but weakly positive in type IIa(r＝0.459). HDL-CHO showed no correlation with sTCHO and sTG. The correlation of CHO and TG with each subfraction was strongly positive in VLDL(r＝0.910), weakly positive(r＝0.49) in LDL, and showed the no correlation in HDL in all cases. These correlation varied in lipidemia types, IIb and IV were strongly positive(r＝0.886, 0.838) in VLDL subfraction, but nomolipidemia cases(r＝0.555) showed sig-nificant weaker correlation(p＜0.0001). In the LDL subfraction, IIb and IV showed no correlation(r＝0.009, 0.163) between CHO and TG. The CHO/TG ratio of three subfractions were widely distributed , and type IIb and IV distributed to lower range than normolipidemia and/or type IIa lipidemia in three subfractions. From these results, dual measurement of LDL-CHO and LDL-TG are useful to estimate qualitative change in the LDL subfraction. For instance, in high LDL-CHO or LDL-TG with low CHO/TG cases, we could suspect the presence of IDL-rich particles in the LDL subfraction or small particle LDL cases. HDL-CHO and CHO/TG show positive(r＝0.714), HDL-CHO and VLDL-TG, VLDL-CHO showed weakly negative correlations(r＝0.500, 0.487), showing that high HDL-CHO level cases tended to have a CHO rich, and low VLDL-TG concentration.
These results indicated that qualitative change in lipoproteins could be clarified by measurement of the TG concentration, in addition to CHO concentrations in three subfractions. We conclude that AG-CHO, TG staining method is useful for diagnosis and monitoring of dyslipidemia.
[Rinsho Byori 50 : 605〜612, 2002]
*1Department of Clinical Pathology, Juntendo University School of Medicine, Bunkyo-ku Tokyo 113-8421
【Key Words】lipoprotein subfraction(リポ蛋白分画)，CHO and TG dual staining(コレステロール，トリグリセライド二重染色)，agarose gel electrophoresis(アガロース電気泳動)
受付2001年12月10日・受理2002年5月16日
*1順天堂大学医学部臨床病理学，*2同 臨床検査部(〒113-8421 東京都文京区本郷2-1-1)
E-mail :yunamine@med.juntendo.ac.jp