原　　著

デジタル画像を用いたリンパ球核構造の
フラクタル次元の測定

丹　羽　欣　正*1　吉　田　　　崇*2　森　分　和　也*3
岡　本　貴　史*4　倉　本　智津子*5　山　崎　英　子*6
松　田　多恵子*7　増　谷　喬　之*8　岡　本　康　幸*9
　　　　　　　　　　　　　　　　　　　　　　
Determination of Fractal Dimension Using Digital Nuclear Image of Lymphocyte

Yoshimasa NIWA*1, Takashi YOSHIDA*2, Kazuya MORIWAKE*3, Takashi OKAMOTO*4,
Chizuko KURAMOTO*5, Eiko YAMAZAKI*6, Taeko MATSUDA*7,
Takayuki MASUTANI*8 and Yasuyuki OKAMOTO, MD*9

Microscopic morphological findings in smeared and stained blood cells are difficult to be characterized quantitatively. However, a recent progress in digital image processing has been enabled to express some of these findings quantitatively. In this report, we propose the usefulness to determine "box" fractal dimension of nuclear image of lymphocyte. Fractal dimension was determined in digital 256-grayscale images of normal, atypical and leukemic lymphocytes by a box-counting method after extracting a nuclear image out of a cellular image, binalyzing and thinning it. The results suggest that fractal dimensions of nuclear images are mainly correlated with changes in chromatin appearance. Fractal dimension could be a useful quantitative parameter for cytological differentiation between normal and atypical lymphocytes.
[Rinsho Byori 50 : 702〜705, 2002]

*9Central Clinical Laboratory, Nara Medical University Hospital, Kashihara 634-8522

【Key Words】image analysis(画像解析)，fractal dimension(フラクタル次元)，lymphocyte(リンパ球)，chromatin appearance(クロマチン構造)

受付2002年2月8日・受理2002年6月10日
*1〜9奈良県立医科大学附属病院中央臨床検査部(〒634-8522 橿原市四条町840)

E-mail :yasuyuki@naramed-u.ac.jp