Immunophenotyping of hematopoietic malignancies is representative application
of cell(surface) marker analysis by flow cytometry and monoclonal antibodies
in the clinical laboratory. The multitude of available monoclonal antibodies
demands a standardization of the selection and combination of antibodies.
Therefore, some international committee or working group proposed the panels
or guidelines for the selection of antibodies. Intracellular antigens are
of major importance for immunophenotyping of hematological malignancies,
and flow cytometric detection of intracellular antigens was improved by
the development of new permeabilization/fixation solutions. Recently new
gating method was recommended for better isolating the target cells in
the flow cytometric analysis. Finally CD55 and CD59 assay for the diag-nosis
of PNH was mentioned.
[Rinsho Byori 50 : 753～760, 2002]
*Department of Clinical laboratory, Tokyo Metropolitan Koto Geriatric Medical Center, Koutou-ku, Tokyo 136-0075
【Key Words】immunophenotyping(イムノフェノタイピング)，flow cytometry(フローサイトメトリー)，hematological malignancies(造血器腫瘍)，antibody panels(抗体パネル)，intracellular antigens(細胞内抗原)，
*東京都江東高齢者医療センター臨床検査科(〒136-0075 東京都江東区新砂3-3-20)
E-mail :naotakes@med.juntendo.ac.jp