技　　　術

ホルマリン固定パラフィン包埋切片を用いたマイクロ
ウェーブ fluorescence in situ hybridization(FISH)法
の実用化への検討

緇　莊　和　子*1　村　上　　　茂*2　陣　内　睦　男*3　木　場　洋　行*4
今　井　優　子*5　山　田　　　孝*6　上　田　善　彦*7　森　　　吉　臣*8
　　　　　　　　　　　　　　　　　　　　　　
Investigation of Practical Application of Fluorescence in situ Hybridization(FISH)
Analysis using Microwave Irradiation in Formalin-fixed,
Paraffin-embedded Tissue Sections

Kazuko KUROSOU, PhD*1, Shigeru MURAKAMI*2, Mutsuo JINNAI*3,
Hiroyuki KOBA*4, Yuuko IMAI, DD*5, Takashi YAMADA, DD*6,
Yoshihiko UEDA, MD*7 and Yoshiomi MORI, MD*8

We investigated fluorescence in situ hybridization(FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections of breast fibroadenoma. Higher percentage of cells with 2 signal copies of chromosome 3 centromere could be obtained in the condition of 5mm thick sections, when we counted cells of more than 4mm of nuclei in thickness. This method showed about the same results as FISH using cells separated from the same tissues. Percentage of cells with 2 signal copies of chromosome 17 centromere in 14 cases was 80.6±4.0%(Mean±S.D.). This method is expected in the application of the prognosis estimation of the breast cancer.
[Rinsho Byori 50 : 830〜834, 2002]

*1Department of Pathology, Dokkyo University School of Medicine, Koshigaya Hospital, Koshigaya 343-8555

【Key Words】fluorescence in situ hybridization : FISH(蛍光 in situ 分子雑種法)，breast fibroadenoma(乳腺線維腺腫)，microwave : MW(マイクロウェーブ)，formalin-fixed(ホルマリン固定)，paraffin-embedded tissues(パラフィン包埋組織)

受付2002年4月19日・受理2002年7月11日
*1,2,4〜8獨協医科大学越谷病院病理部，*3,8同　医学総合研究所(〒343-8555 越谷市南越谷2-1-50)
*1東京医科大学臨床検査医学講座(〒160-0023 新宿区西新宿6-7-1)

E-mail :kurosou@dokkyomed.ac.jp