[Rinsho Byori 50 : 830～834, 2002]

Investigation of Practical Application of Fluorescence in situ Hybridization(FISH)
Analysis using Microwave Irradiation in Formalin-fixed,
Paraffin-embedded Tissue Sections

Kazuko KUROSOU, PhD*1, Shigeru MURAKAMI*2, Mutsuo JINNAI*3,
Hiroyuki KOBA*4, Yuuko IMAI, DD*5, Takashi YAMADA, DD*6,
Yoshihiko UEDA, MD*7 and Yoshiomi MORI, MD*8

We investigated fluorescence in situ hybridization(FISH) analysis using microwave irradiation in formalin-fixed, paraffin-embedded tissue sections of breast fibroadenoma. Higher percentage of cells with 2 signal copies of chromosome 3 centromere could be obtained in the condition of 5mm thick sections, when we counted cells of more than 4mm of nuclei in thickness. This method showed about the same results as FISH using cells separated from the same tissues. Percentage of cells with 2 signal copies of chromosome 17 centromere in 14 cases was 80.6±4.0%(Mean±S.D.). This method is expected in the application of the prognosis estimation of the breast cancer.

*1Department of Pathology, Dokkyo University School of Medicine, Koshigaya Hospital, Koshigaya 343-8555

【Key Words】fluorescence in situ hybridization : FISH, breast fibroadenoma, microwave : MW, formalin-fixed, paraffin-embedded tissues