原　　　著

フローサイトメトリーを利用した CD5 半定量による
Diffuse Large B-cell Lymphoma 細分類の試み

真　鍋　紀　子*1 　山　岡　源　治*2 　大　西　宏　明*3 　荒　井　　　健*4
中　石　浩　己*5 　梶　川　達　志*6 　窪　田　良　次*7 　田　中　輝　和*8
北　中　　　明*9 　脇　　　正　人*10　川　上　公　宏*11　永　井　雅　巳*12
石　田　俊　彦*13　田　港　朝　彦*14　　　　　　　　　　　　　　　　　　
　　　　　　　　　　　　　　　　　　　　　　
Sub-Classification of Diffuse Large B-Cell Lymphoma
by Semi-Quantification of the CD5 Expression with Flow Cytometric Analysis

Noriko MANABE*1, Genji YAMAOKA*2, Hiroaki OHNISHI, MD*3, Takeshi ARAI*4,
Hiromi NAKAISHI*5, Tatushi KAJIKAWA*6, Yoshitsugu KUBOTA, MD*7, Terukazu TANAKA, MD*8,
Akira KITANAKA, MD*9, Masato WAKI, MD*10, Kimihiro KAWAKAMI, MD*11,
Masami NAGAI, MD*12, Toshihiko ISHIDA, MD*13 and Tomohiko TAMINATO, MD*14

CD5＋diffuse large B-cell lymphoma(CD5^＋DLBCL) is known to have different characteristics than CD5⁻DLBCL and mantle cell lymphoma(MCL). 9 patients with CD5^＋DLBCL were reviewed, and the results were compared with those of 8 CD5⁻DLBCL and 3 cyclin D1^＋MCL patients. CD5^＋DLBCL was more closely related to many aggressive clinical features or parameters than CD5⁻DLBCL : 67% of the patients were older than 60 years, 67% with performance status ≧2, 89% with serum lactate dehydrogenase level higher than normal, 78% with stage III/IV disease at diagnosis, and 78% with more than one extranodal lesion. The overall International Prognostic Index score for the patients with CD5^＋DLBCL was thus sig-nificantly higher than that for those with CD5⁻DLBCL.
Immunophenotypically, CD5^＋DLBCL was characterized by CD5^＋CD10⁻CD19^＋CD20^＋CD21⁻CD23⁻cyclin D1⁻phenotype and the predominant expression of surface IgM. Of particular interest is that the survival rate of CD5^＋DLBCL patients was significantly inferior to that of patients with CD5⁻DLBCL. To further characterize CD5^＋DLBCL, we semi-quantified the CD5 expression in DLBCL cells. Our findings suggest that CD5^＋DLBCL may constitute a unique subgroup of DLBCL and, moreover, CD5^＋DLBCL may consist of several subgroups.
[Rinsho Byori 50 : 906〜911, 2002]

*1Department of medical technology Kagawa Prefectural College of health sciences, Kida-gun, kagawa-pref. 761-0123

受付2002年5月31日・受理2002年8月13日
*1香川県立医療短期大学臨床検査学科(〒761-0123 香川県木田郡牟礼町大字原281番地1)
*2,4〜6香川医科大学医学部附属病院検査部，*3,14同　臨床検査医学，*7同　輸血部，*8同　環境保健科学，
*9〜13同　第一内科(〒761-0793 香川県木田郡三木町池戸1750-1)

E-mail :manabe@chs.pref.kagawa.jp