原　　　著

抗 p53 抗体の腫瘍マーカーとしての臨床的意義

酒　井　　　寛*1　岡　本　英　一*2
　　　　　　　　　　　　　　　　　　　　　　
Clinical Importance of Serum anti-p53 Antibodies as Tumor Markers

Hiroshi SAKAI, PhD*1 and Eiichi OKAMOTO, MD*2

Anti-p53 antibodies are autoantibodies induced by mutation of p53 cancer-suppressor gene, and are considered to be indirect markers for p53 gene mutations and abnormally high p53 gene levels.
We evaluated the usefulness of the measurement of anti-p53 antibodies by enzymed-linked immunosorbent assay using serum samples from patients with various disorders and normal subjects.
The anti-p53 antibody concentration was high in patients with lung, esophageal, gastric, hepatocellular, colonic, rectal or ovarian cancer and significantly differed between the group with neoplasms and those with non-neoplastic disorders. Particularly high concentrations were observed in patients with malignant tumors.
The mean agreement rate between anti-p53 antibodies and conventional tumor markers was only 47.8% despite slight differences among disorders. The positive rate increased to 63.0% by their combination assay. In addition, anti-p53 antibodies were independent markers, not complimentary to conventional markers. The mean agreement rate between anti-p53 antibodies and tissue p53 was 70.0%. Though the anti-p53 antibody-positive rate was lower than the tissue p53-positive rate, anti-p53 antibodies may be useful new tumor markers because specimens from the affected tissue are not necessary.
[Rinsho Byori 50 : 970〜975, 2002]

*1Department of Clinical Laboratory, Kyoto National Hospital, Kyoto 612-8555

【Key Words】malignant neoplastic diseases(悪性腫瘍)，anti-p53 antibodies(抗 p53 抗体)，tumor marker(腫瘍マーカー)，enzyme-linked immunosorbent assay(ELISA 法)

受付2002年3月27日・受理2002年9月20日
*1,2国立京都病院臨床検査科(〒612-8555 京都市伏見区深草向畑町1-1)
*1(現)国立療養所兵庫中央病院･研究検査科(〒669-1515 三田市大原1314)

E-mail :sakai@hyougotyu.hosp.go.jp