血液培養ボトルからの結核菌群迅速同定法
－抗 MPB64 抗体免疫クロマトグラフィー法
判定時の H2O2 添加の試み－
阿 部 桂 子*1 原 正 道*2
Improvement for Identification of Mycobacterium tuberculosis Complex by
Immunochromatographic Assay Anti-MPB 64 Monoclonal Antibodies
with "H2O2" Addition Trial on Blood Culture Samples
Keiko ABE*1 and Masamichi HARA, MD, PhD*2
An improvement trial for identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using anti-MPB 64 monoclonal antibodies(MPB
64-ICA) is documented.
The new MPB 64-ICA is very useful for simple and rapid identification of the Mycobacterium tuberculosis complex with specificity, but it is a little difficult to prove the Mycobacterium tuberculosis complex on the blood culture samples taken from the immunosuppressive or the AIDS patients, because the reactive bands are red or reddish-purple in both the control and positive zones of immunochromatographic assay, which are often indistinguishable from the background mixed with hemoglobin caused from the blood samples.
We tried to remove the hemoglobin interference in the samples and was able to make clear the back-ground with addition of hydrogen peroxide(H2O2).The results are followings ; the optimum time and density of H2O2 are from 5 to 15 minutes and 35%, respectively.
Accordingly, the improvement trial for identification of the Mycobacterium tuberculosis complex by immunochromatographic assay using MPB64-ICA with H2O2 is useful to identify the positive reactive band from the interference by hemoglobin.
[Rinsho Byori 50 : 1067～1071, 2002]
*1Department of Clinical Laboratory, National Sanatorium Minami Yokohama Hospital, Yokohama 233-8503
【Key Words】blood culture samples(血液培養検体)，Mycobacterium tuberculosis complex(結核菌群)，immunochromatographic assay(免疫クロマトグラフィー法)，Anti-MPB64 monoclonal antibodies(抗 MPB64 抗体)，H2O2 addition(過酸化水素水の添加)
受付2002年6月24日・受理2002年8月27日
*1国立療養所南横浜病院研究検査科(〒233-8503 横浜市港南区芹が谷2-6-1)
*2横浜市立大学医学部附属病院・病院長(〒236-0004 横浜市金沢区福浦3-9)
E-mail :kei-abe@mub.biglobe.ne.jp