マイクロサテライトのフラグメント解析法
を用いた多胎の卵性判定
久保田 紀 子*1 海 野 信 也*2 小木曽 嘉 文*3
DNA Typing for Determination of Zygosity on Multiple Pregnancies
Using Fragment Analysis for Microsatellite
Noriko KUBOTA*1, Nobuya UNNO, MD*2 and Yoshifumi OGISO, MD*3
Zygosity on multiple pregnancies is important factor of prognosis in fetus and new bones. In the past, determination of zygosity has been made mainly on the basis of histological and microscopic observations. Such a morphological distinction, however is not sufficient.
Fragment analysis based on capillary electrophoresis has been developed
as a rapid and accurate technique for DNA typing. To elucidate the clinical
availability of fragment analysis, we examined the zygosity of nine multiple
pregnancies (three twins and six triplets) by fragment analysis of microsatellite
DNAs and other common DNA tests(PCR-SSCP assay and VNTR analysis using
agarose gel electrophoresis). Fragment analysis and PCR-SSCP identified
the heterozygosity in two of three twins and all of six triplets including
one presumable dizygotic triplet, while VNTR analysis failed to recognize
one of two dizygotic twins and three of five trizygotic triplets. Although
detection efficiency of PCR-SSCP seems to be similar to that of fragment
analysis, PCR-SSCP could not separate the several microsatellite DNAs that
fragment analysis showed to be heterozygous. Moreover, fragment analysis
could detect a minor population possessing the different DNA types(＞5%)
in the same cell suspension.
The present results suggest that fragment analysis of microsatellite DNAs may be an excellent and beneficial method for clinical application of zygosity determination in multiple pregnancies.
[Rinsho Byori 50 : 1079〜1084, 2002]
*1Division of Clinical Pathology, Nagano Children's Hospital, Toyoshina 399-8255
【Key Words】Microsatellite(マイクロサテライト)，fragment analysis(フラグメント解析法)，DNA
typing(DNA タイピング)，zygosity of multiple pregnancies(卵性)
受付2002年5月2日・受理2002年10月23日
*1,3長野県立こども病院臨床病理科，*2同 産科(〒399-8255 長野県南安曇郡豊科町豊科3100)