原著

免疫法による尿中ヘモグロビンの定量法

渡　辺　信　子*1　橋　本　佳　明*2　二　村　　　梓*3
真　重　文　子*4　中　原　一　彦*5 　　 　 　　　 　
　　　　　　　　　　　　　　　　　　　　　　
Quantification of Urinary Hemoglobin by an Immunological Method

Nobuko WATANABE*1, Yoshiaki HASHIMOTO, MD*2, Azusa FUTAMURA*3,
Fumiko MASHIGE*4 and Kazuhiko NAKAHARA, MD*5

The quantification method of urinary hemoglobin has not been established. We examined whether a reagent(Eiken, Tokyo) used to immunologically assay fecal hemoglobin could be utilized to quantify urinary hemoglobin. The coefficients of variation were 2.2〜3.0% in the reproducibility of one-run assays using urine and 4.3〜5.7% in that of multiple-run assays using the standard sample of hemoglobin. Urinary hemoglobin was unstable and decreased in a time- and temperature-dependent manner. However, addition of the hemoglobin stabilization buffer(50mM phosphate buffer, pH6.4)(Eiken) to urine made urinary hemoglobin stable. Urinary hemoglobin levels did not change significantly when stored at 4℃ for 7 days or at −80℃ for 30 days. The hemoglobin concentration(mean±SD) of urine showing 1＋, 2＋ and 3＋ with a test strip was 385±165(n＝30), 1070±499(n＝40) and 4130±2770ng/ml(n＝20), respectively. Urinary hemoglobin did not correlate with urinary albumin, transferrin, immunoglobulin G, N-acetyl-β-D-glucosaminidase nor α 1 microglobulin. These results suggest that this immunological method using the hemoglobin stabilization buffer can be utilized for quantification of urinary hemoglobin, which may provide clinically important information.
[Rinsho Byori 51 : 403〜408, 2003]

*1Department of Clinical Laboratory Medicine, The University of Tokyo Hospital, Bunkyo-ku, Tokyo 113-8655

【Key Words】urinary hemoglobin(尿中ヘモグロビン)，immunological assay(免疫学的測定法)，quantification of urinary hemoglobin(尿中ヘモグロビン定量)，chronic glomerulonephritis(慢性糸球体腎炎)

受付2002年9月26日・受理2003年3月19日
*1〜5東京大学医学部附属病院検査部(〒113-8655 東京都文京区本郷7-3-1)