関節リウマチ患者に認められた
IgG3-κ 型 M蛋白の抗DNA抗体の性状
佐 藤 裕 久*1 寺 沢 儀 男*2 貝 森 光 大*3
竹 森 弘 光*4 大 竹 皓 子*5 櫻 林 郁之介*6
Characterization of Anti-DNA Antibody on IgG3-κ type M-protein
in a Patient with Rheumatoid Arthritis
Hirohisa SATO*1, Norio TERASAWA*2, Mitsuomi KAIMORI, MD*3,
Hiromitsu TAKEMORI, MD*4, Teruko OTAKE*5 and Ikunosuke SAKURABAYASHI, MD, PhD*6
A 66 year-old male patient with rheumatoid arthritis, was admitted due to bronchial pneumonia. Anti-nuclear antibody and anti-DNA antibody were both detected in the patient’s serum and many LE-like cells were found in the pleural effusion. In addition, M-proteins(IgG-k type，IgM-k type) were detected in both serum and pleural effusion by immunofixation electrophoresis.
Purified IgG fraction by gel filtration(Superdex 200HR) showed high titer of anti-nuclear antibody(×160) and anti-DNA(39.2 AU/ml) antibody. Furthermore, anti-DNA antibody was detected in the fraction III(fraction of M-protein) by ion-exchange chromatography.
In conclusion, these results indicate that M-protein(IgG3-k type) contains anti-DNA antibody and it is suggests that M-protein induces the production of LE like cell in this case.
[Rinsho Byori 51 : 627〜632, 2003]
*1Department of Clinical Pathology, Aomori Prefectural Central Hospital, Aomori 030-8553
【Key Words】M-protein(M 蛋白)，IgG3-k type(IgG3-k 型)，LE-like cell(LE 様細胞)，Anti-nuclear antibody(抗核抗体)，Anti-DNA antibody(抗 DNA 抗体)
受付2003年2月13日・受理2003年4月17日
*1〜3青森県立中央病院臨床検査部，*4同 リウマチ・血液内科(〒030-8553 青森市東造道2-1-1)
*5慶応義塾大学医学部中央臨床検査部(〒160-8582 東京都新宿区信濃町35)
*6自治医科大学大宮医療センター臨床病理部(〒330-8503 さいたま市天沼1-847)
E-mail :hsatoh@r66.7-dj.com